2-Keto-D-Glucose, also known as D-Glucosone, is primarily utilized in biochemical research and industrial applications. It serves as a key intermediate in the synthesis of various organic compounds, including pharmaceuticals and specialty chemicals. In the food industry, it is explored for its potential as a low-calorie sweetener due to its unique metabolic properties. Additionally, it is studied in enzymatic processes and metabolic pathways to understand its role in carbohydrate metabolism. Its applications also extend to the development of biosensors and diagnostic tools, where it aids in detecting specific enzymatic activities.