N-Acetyl-2,3-didehydro-2-deoxyneuraminic Acid is primarily utilized in biochemical research as a key intermediate in the study of sialic acid metabolism. It plays a significant role in understanding enzymatic processes, particularly those involving sialidases and sialyltransferases, which are crucial in cellular communication and pathogen interactions. This compound is also used in the development of inhibitors for viral neuraminidases, making it valuable in antiviral drug research. Additionally, it aids in the synthesis of sialic acid derivatives, which are important for investigating glycobiology and developing therapeutic agents targeting glycoproteins and glycolipids.