2-Azidoethyl β-D-Glucopyranoside is primarily used in biochemical and biotechnological research as a versatile building block for glycoconjugate synthesis. Its azido group allows for efficient click chemistry reactions, particularly copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling the attachment of various biomolecules or probes. This compound is valuable in glycobiology for studying carbohydrate-protein interactions, designing glycoconjugate vaccines, and developing targeted drug delivery systems. It also serves as a precursor for synthesizing glycosylated materials, such as glycoarrays or glycopolymers, which are essential tools for investigating cell surface recognition processes and developing diagnostic assays. Additionally, its application extends to the development of bioorthogonal labeling strategies, facilitating the visualization and tracking of glycans in complex biological systems.