D-(-)-Cellobiose is widely used in biochemical research as a substrate to study the activity of cellulase enzymes, which are crucial for breaking down cellulose into simpler sugars. It serves as a model compound to understand cellulose degradation processes, aiding in the development of efficient biofuel production methods. Additionally, it is utilized in the food industry as a low-calorie sweetener and a prebiotic to promote gut health by stimulating the growth of beneficial bacteria. In the pharmaceutical sector, it is employed as an excipient in drug formulations to enhance stability and bioavailability. Its role in material science includes acting as a precursor for synthesizing biodegradable polymers and other advanced materials.