Used as a stable isotope-labeled internal standard in mass spectrometry and nuclear magnetic resonance (NMR) studies to trace metabolic pathways such as glycolysis and the pentose phosphate pathway. Its deuterated form allows precise quantification and monitoring of glucose metabolism in biological systems, including cell cultures, tissues, and in vivo models. Commonly applied in metabolic flux analysis, pharmacokinetic studies, and clinical diagnostics to distinguish exogenous from endogenous glucose contributions. Also employed in isotopic labeling experiments to study enzyme mechanisms and carbohydrate transformations in biochemical research.