Anhydroicaritin is primarily studied for its potential biological and pharmacological applications, particularly in the field of cancer research. It exhibits anti-tumor activity by inducing apoptosis and inhibiting the proliferation of various cancer cell lines, including those of breast, liver, and leukemia cancers. The compound has shown the ability to modulate key signaling pathways such as PI3K/AKT and MAPK, which are involved in cell survival and growth. It also possesses anti-inflammatory and antioxidant properties, making it a candidate for development in treating inflammatory diseases and oxidative stress-related conditions. Additionally, anhydroicaritin has been investigated for its neuroprotective effects, suggesting possible use in neurodegenerative disorders. Due to its phytoestrogenic activity, it may influence bone metabolism and has been explored for potential application in osteoporosis management. Research is ongoing to improve its bioavailability and therapeutic efficacy through formulation strategies.