AKR1C1-IN-1 is primarily used in research settings to study the role of the AKR1C1 enzyme in various biological processes. It is applied as a selective inhibitor to investigate the enzyme's involvement in hormone metabolism, particularly in the regulation of steroid hormones like progesterone and estradiol. This compound is valuable in understanding the enzyme's impact on cancer progression, as AKR1C1 is often overexpressed in hormone-dependent cancers such as breast and prostate cancer. Researchers use AKR1C1-IN-1 to explore potential therapeutic strategies by targeting the enzyme to disrupt cancer cell growth and hormone signaling pathways. Additionally, it aids in studying drug resistance mechanisms, as AKR1C1 is implicated in the metabolism of certain chemotherapeutic agents. Its application extends to developing novel treatments for conditions influenced by steroid hormone dysregulation.