Bicine is widely used as a buffering agent in biochemical and molecular biology applications. It is particularly effective in maintaining stable pH levels in the range of 7.6 to 9.0, making it suitable for various enzymatic reactions and protein studies. Bicine is commonly employed in electrophoresis, chromatography, and cell culture media due to its low metal ion binding capacity, which minimizes interference with biological processes. Additionally, it is utilized in the preparation of solutions for plant tissue culture and in the study of photosynthesis, where it helps stabilize the pH of chloroplast suspensions. Its compatibility with a wide range of biochemical assays and its ability to maintain pH stability under varying conditions make Bicine a valuable reagent in research laboratories.