Glycylglycine buffer is widely used in biochemical and biophysical research due to its effective buffering capacity in the pH range of 7.5 to 8.5. It is particularly valuable in enzyme studies, where maintaining a stable pH is crucial for accurate results. This buffer is also employed in protein purification processes, as it helps maintain the integrity and functionality of proteins during extraction and separation. Additionally, glycylglycine buffer is utilized in cell culture applications to provide a stable environment for cell growth and metabolic activity. Its compatibility with various biochemical assays makes it a preferred choice in studies involving nucleic acids, antibodies, and other biomolecules. Furthermore, it is used in spectroscopic techniques, such as NMR and X-ray crystallography, to ensure optimal conditions for sample analysis.