Glycylglycine buffer is widely used in biochemical and biophysical research due to its effective buffering capacity in the pH range of 7.5 to 8.5. It is particularly valuable in enzyme studies, where maintaining a stable pH is crucial for accurate results. This buffer is also employed in protein crystallization processes, as it helps create optimal conditions for crystal growth. Additionally, glycylglycine buffer is utilized in cell culture applications to maintain a consistent environment for cell growth and viability. Its compatibility with various biochemical assays makes it a preferred choice in laboratories for studying enzymatic reactions and protein interactions.