Glycine buffer in PBS is widely used in biological and biochemical research for stabilizing pH in various experimental conditions. It is particularly effective in protein purification processes, such as affinity chromatography, where it helps maintain a consistent pH environment, ensuring protein stability and functionality. Additionally, it is utilized in immunoassays, including ELISA, to optimize antibody-antigen interactions by providing a stable buffering system. In cell culture applications, glycine buffer in PBS supports cell growth and viability by maintaining physiological pH levels. It also plays a role in electrophoresis techniques, where it assists in the separation of proteins and nucleic acids by controlling pH during the process. Furthermore, it is employed in diagnostic assays and vaccine development to ensure accurate and reproducible results.