MOPS Buffer is widely used in biological and biochemical research due to its ability to maintain a stable pH in the range of 6.5 to 7.9. It is commonly employed in electrophoresis, particularly for RNA separation in agarose gels, as it provides a consistent pH environment that minimizes RNA degradation. In cell culture, MOPS Buffer is utilized to maintain optimal pH levels, ensuring healthy cell growth and metabolic activity. It is also a key component in various enzymatic assays and protein studies, where precise pH control is critical for accurate results. Additionally, MOPS Buffer is used in chromatography and other analytical techniques to stabilize samples and maintain the integrity of biological molecules during analysis. Its low reactivity with metal ions makes it suitable for use in experiments involving metal-sensitive enzymes or proteins.