MES hydrate is widely used as a buffering agent in biochemical and molecular biology applications. It is particularly effective in maintaining stable pH conditions in cell culture media, electrophoresis, and protein purification processes. Its buffering capacity is optimal in the pH range of 5.5 to 6.7, making it suitable for experiments involving enzymes, nucleic acids, and other pH-sensitive biomolecules. Additionally, MES hydrate is utilized in the formulation of cosmetics and personal care products to stabilize pH and enhance product performance. Its low toxicity and compatibility with biological systems also make it a preferred choice in pharmaceutical research and development.