MOPS buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in electrophoresis, particularly for RNA agarose gel electrophoresis, as it provides stable pH conditions and minimizes RNA degradation. MOPS is also utilized in cell culture applications to maintain optimal pH levels for cell growth and viability. Additionally, it serves as a buffering agent in protein purification processes and enzyme assays, ensuring consistent reaction environments. Its low reactivity with metal ions makes it suitable for use in studies involving metal-sensitive enzymes or proteins. MOPS buffer is also applied in nucleic acid hybridization techniques, where it helps maintain pH stability during hybridization and washing steps.