MOPS Buffer is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain a stable pH environment, ensuring optimal growth conditions for cells. Additionally, MOPS Buffer is utilized in electrophoresis, particularly for RNA separation, as it provides consistent pH and minimizes degradation of RNA molecules. It is also applied in protein purification processes and enzymatic assays, where maintaining a stable pH is critical for accurate results. Furthermore, MOPS Buffer is used in molecular biology techniques, including Northern blotting, to ensure reliable and reproducible outcomes. Its low toxicity and compatibility with various biological systems make it a preferred choice in laboratory applications.