MOPS Buffer is widely used in biological and biochemical research due to its effective buffering capacity in the pH range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain stable pH conditions, ensuring optimal growth and viability of cells. In molecular biology, MOPS Buffer is a key component in electrophoresis, particularly for RNA separation in agarose gels, as it provides a stable environment that minimizes RNA degradation. It is also utilized in protein purification processes to maintain consistent pH during chromatography steps. Additionally, MOPS Buffer is suitable for use in enzyme assays and other biochemical reactions where pH stability is critical for accurate results. Its low UV absorbance makes it ideal for applications involving spectrophotometric analysis.