MOPS buffer is widely used in molecular biology and biochemistry for maintaining stable pH conditions in various experimental procedures. It is particularly effective in electrophoresis, where it helps to separate nucleic acids like RNA and DNA with minimal degradation. In cell culture, MOPS buffer provides a consistent environment for cell growth and metabolic studies. Additionally, it is utilized in protein purification processes to ensure optimal pH for enzyme activity and protein stability. Its low reactivity with metal ions makes it suitable for use in metal-sensitive assays. MOPS buffer is also employed in spectroscopy and chromatography to maintain pH without interfering with analytical results.