MOPSO buffer is widely used in biochemical and molecular biology research for maintaining stable pH levels in various experimental conditions. It is particularly effective in cell culture applications, where it helps to create an optimal environment for cell growth and viability. The buffer is also utilized in electrophoresis techniques, such as SDS-PAGE, to ensure consistent and reliable separation of proteins. Additionally, MOPSO is employed in enzymatic assays and protein purification processes, where precise pH control is critical for maintaining enzyme activity and protein stability. Its low toxicity and compatibility with biological systems make it a preferred choice for in vitro studies and diagnostic applications.