MES buffer is widely used in biochemical and molecular biology research due to its effective buffering range of pH 5.5 to 6.7. It is commonly employed in electrophoresis, particularly for separating nucleic acids and proteins, as it provides stable pH conditions. MES buffer is also utilized in cell culture applications to maintain optimal pH levels for cell growth and viability. Additionally, it serves as a buffering agent in chromatography, ensuring consistent separation and analysis of biomolecules. Its low UV absorbance makes it suitable for spectrophotometric assays, where minimal interference is crucial. MES buffer is also used in plant tissue culture to support healthy plant growth under controlled pH conditions. Its compatibility with various enzymes and biochemical reactions further enhances its utility in laboratory settings.