MES buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 5.5 to 6.7. It is commonly employed in electrophoresis, particularly for nucleic acid separation, as it provides stable pH conditions during the process. Additionally, MES buffer is utilized in cell culture applications to maintain optimal pH levels for cell growth and viability. It is also a key component in protein purification and crystallization, where precise pH control is essential. In plant biology, MES buffer is often used in hydroponic systems to regulate pH and support nutrient uptake. Its compatibility with various biochemical assays makes it a versatile choice for laboratory experiments.