MES buffer is widely used in biochemical and molecular biology applications due to its effective buffering range of pH 5.5 to 6.7. It is commonly employed in electrophoresis, particularly for separating proteins and nucleic acids, as it provides stable pH conditions during the process. MES is also utilized in cell culture media to maintain optimal pH levels for cell growth and viability. Additionally, it serves as a buffer in chromatography techniques, such as ion exchange and size exclusion chromatography, to ensure consistent separation and analysis of biomolecules. Its low UV absorbance makes it suitable for spectrophotometric assays and protein purification processes. MES buffer is also used in plant tissue culture and enzyme studies, where precise pH control is critical for experimental accuracy.