HEPES buffer is widely used in biological and biochemical research due to its ability to maintain a stable pH in cell culture media and enzymatic reactions. It is particularly effective in experiments involving live cells, as it does not interfere with cellular processes or membrane integrity. HEPES is commonly employed in protein studies, electrophoresis, and tissue culture, where consistent pH levels are critical. Additionally, it is utilized in molecular biology techniques like PCR and DNA sequencing to ensure optimal conditions for enzyme activity. Its low toxicity and minimal interaction with metal ions make it a preferred choice for various laboratory applications.