CHES buffer is widely used in biochemical and molecular biology research due to its effective buffering range at higher pH levels, typically between 8.6 and 10.0. It is particularly useful in enzyme studies and protein purification processes where maintaining a stable alkaline pH is critical. CHES buffer is also employed in electrophoresis techniques, such as SDS-PAGE, to ensure optimal separation of proteins. Additionally, it is utilized in cell culture applications to maintain pH stability in growth media. Its compatibility with various biochemical assays makes it a valuable tool in studying enzymatic reactions and protein interactions under alkaline conditions.