CHES buffer is widely used in biochemical and molecular biology research due to its effective buffering range between pH 8.6 and 10.0. It is particularly useful in enzymatic assays and protein studies where alkaline conditions are required. For instance, it is employed in the study of alkaline phosphatase, an enzyme that functions optimally at high pH levels. Additionally, CHES buffer is utilized in electrophoresis techniques, such as SDS-PAGE, to maintain stable pH conditions during protein separation. Its stability at higher temperatures also makes it suitable for applications involving heat-sensitive biochemical reactions. In cell culture, CHES buffer helps maintain the pH of media, ensuring optimal growth conditions for certain cell types. Furthermore, it is used in the preparation of samples for electron microscopy to preserve structural integrity under alkaline conditions.