CAPS buffer is widely used in biochemical and molecular biology applications due to its effective pH range of 9.7 to 11.1. It is particularly useful in protein electrophoresis, especially for separating low molecular weight proteins, as it provides stable pH conditions during the process. CAPS buffer is also employed in Western blotting, where it facilitates the transfer of proteins from gels to membranes with high efficiency. Additionally, it is utilized in enzyme assays and other biochemical reactions that require alkaline conditions. Its compatibility with various biochemical techniques makes it a versatile choice for researchers working in protein analysis and related fields.