CAPS buffer is widely used in biochemical and molecular biology applications due to its effective buffering range at higher pH levels, typically between 9.7 and 11.1. It is commonly employed in protein electrophoresis, particularly in SDS-PAGE, where it helps maintain a stable pH environment for protein separation. CAPS buffer is also utilized in Western blotting as a transfer buffer, ensuring efficient protein transfer from gels to membranes. Additionally, it is used in enzyme assays and other biochemical reactions that require alkaline conditions. Its compatibility with various biological systems makes it a valuable tool in research and diagnostic laboratories.