Bicine buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.6 to 9.0. It is particularly valuable in protein studies, enzyme assays, and electrophoresis, where maintaining a stable pH is critical. Bicine is often employed in the preparation of cell culture media and in chromatography techniques, such as ion-exchange and gel filtration, to ensure optimal separation and analysis of biomolecules. Additionally, it is utilized in plant tissue culture and as a component in buffer systems for studying enzyme kinetics and protein interactions. Its low metal ion chelation properties make it suitable for applications involving metal-sensitive enzymes and proteins.