Bicine buffer is widely used in biochemical and molecular biology research due to its effective buffering capacity in the pH range of 7.6 to 9.0. It is particularly useful in protein studies, enzyme assays, and electrophoresis, where maintaining a stable pH is critical. Bicine is also employed in the preparation of cell culture media and in the crystallization of proteins, as it helps maintain optimal conditions for protein stability. Additionally, it is used in plant tissue culture and as a component in solutions for isolating and purifying nucleic acids. Its low metal ion chelating ability makes it suitable for applications where metal interactions must be minimized.