CAPS is widely used in biochemical and molecular biology applications as a buffering agent. It is particularly effective in maintaining stable pH conditions in the range of 9.7 to 11.1, making it suitable for various experimental procedures. One of its key applications is in protein electrophoresis, where it helps in separating proteins based on their size and charge. CAPS is also utilized in Western blotting, where it assists in the transfer of proteins from gels to membranes, ensuring efficient and accurate detection of target proteins. Additionally, it is employed in enzyme assays and other biochemical reactions that require a slightly alkaline environment. Its compatibility with many biological systems and its ability to provide consistent pH control make CAPS a valuable reagent in research laboratories.