MOPS is widely used as a buffering agent in biological and biochemical research due to its effective pH stabilization in the range of 6.5 to 7.9. It is commonly employed in cell culture media to maintain optimal conditions for cell growth and viability. In molecular biology, MOPS is utilized in electrophoresis, particularly for RNA separation, as it provides a stable environment that minimizes RNA degradation. Additionally, it is used in protein purification processes to maintain the integrity and functionality of proteins. MOPS is also applied in diagnostic assays and enzyme studies, where precise pH control is critical for accurate results. Its low toxicity and compatibility with various biological systems make it a preferred choice in laboratory settings.