MOPS hemisodium salt is a buffering agent effective in the pH range of 6.5-7.9, widely used in biological and biochemical research. It is particularly effective in maintaining stable pH levels in cell culture media, electrophoresis, protein purification processes, and chromatography. Its compatibility with various biological systems makes it a preferred choice for experiments involving enzymes, nucleic acids, and other sensitive biomolecules. Additionally, it is utilized in molecular biology techniques, such as PCR and gel electrophoresis, to ensure optimal conditions for reactions and separations. Its low toxicity and minimal interference with biological processes further enhance its applicability in laboratory settings.