MOPSO is widely used as a buffering agent in biochemical and biological research. It is particularly effective in maintaining stable pH conditions in cell culture media, enzyme assays, and protein purification processes. Its buffering range, typically between pH 6.5 and 7.9, makes it suitable for applications requiring near-neutral pH environments. MOPSO is also utilized in electrophoresis and chromatography techniques due to its minimal interference with biological molecules. Additionally, it is employed in the study of cellular processes and molecular interactions, providing a consistent and reliable pH environment for accurate experimental results.