BODIPY-Cholesterol (BCh2) is widely used in biological research as a fluorescent probe to study cholesterol dynamics in cells. Its application includes visualizing and tracking cholesterol distribution and movement within cellular membranes, providing insights into lipid rafts and membrane organization. BCh2 is particularly valuable in live-cell imaging due to its bright fluorescence and photostability, enabling real-time observation of cholesterol-related processes. It is also employed in studies of cholesterol trafficking, uptake, and efflux, helping to understand diseases linked to cholesterol dysregulation, such as atherosclerosis and neurodegenerative disorders. Additionally, BCh2 serves as a tool in drug discovery to screen compounds that modulate cholesterol metabolism or transport.