This chemical is widely used in bioconjugation and labeling applications, particularly in the field of biochemistry and molecular biology. It serves as a linker molecule, enabling the attachment of biotin to various biomolecules, such as proteins, peptides, or nucleic acids. The biotin moiety allows for strong and specific binding to avidin or streptavidin, which is useful in techniques like ELISA, immunohistochemistry, and fluorescence microscopy. Its PEG (polyethylene glycol) spacer enhances solubility and reduces steric hindrance, improving the efficiency of biotinylation processes. Additionally, it is employed in drug delivery systems to create targeted therapies by conjugating bioactive molecules with biotin for specific cellular uptake.