Used in bioconjugation processes to attach biotin to proteins, peptides, or other molecules for detection and purification in immunoassays and molecular biology techniques. The acetamidoethyl linker reduces non-specific interactions and enhances stability under various conditions. Commonly employed in ELISA, Western blotting, affinity chromatography, and cell imaging due to its strong binding with streptavidin or avidin. Also utilized in diagnostic kits and research tools for labeling and capturing biomolecules with high specificity. Suitable for both in vitro and some ex vivo applications.