Used primarily in research settings as a biomarker for peroxisomal disorders, particularly in diagnosing conditions like Zellweger syndrome where very long-chain fatty acids accumulate due to metabolic defects. It is also utilized in lipidomics studies to understand cellular membrane composition and fatty acid metabolism. Additionally, heptacosanoic acid serves as a reference standard in analytical chemistry for the detection and quantification of long-chain fatty acids in biological samples.