Widely used in cell culture and microbiological media to support growth, especially in organisms that cannot synthesize biotin endogenously. Plays a critical role in enzyme assays and diagnostic kits due to its strong binding affinity to streptavidin and avidin, enabling highly sensitive detection in immunoassays like ELISA and Western blotting. Commonly added to cosmetic formulations for promoting healthy skin, hair, and nails. Used as a nutritional supplement in pharmaceuticals to address biotin deficiency. Employed in molecular biology for labeling, purification, and immobilization of biomolecules through biotin-streptavidin technology. Also utilized in animal feed to improve metabolic function and overall health.