Oil Red O Stain Kit is widely used to detect and visualize neutral lipids and triglycerides in cultured cells. It is commonly applied in research related to adipogenesis, lipid metabolism, and metabolic diseases such as obesity and fatty liver disease. The stain is particularly useful in differentiating adipocytes from other cell types by highlighting lipid droplets in the cytoplasm with a bright red color under a light microscope. This kit is optimized for use in cell culture systems, allowing for reliable and consistent staining in assays involving stem cell differentiation into fat cells or drug screening studies targeting lipid accumulation. The protocol is straightforward, making it suitable for both routine lab use and high-throughput applications.