Apidaecin IB is a proline-rich antimicrobial peptide primarily used in research focused on novel antibacterial agents due to its potent activity against Gram-negative bacteria. It is studied for its ability to combat bacterial infections, including those caused by antibiotic-resistant strains. Its mechanism of action involves intracellular targeting, such as inhibiting bacterial protein synthesis by interacting with ribosomes or chaperone proteins, rather than direct membrane disruption, making it a promising candidate for developing new antibiotics or antimicrobial peptides with low toxicity to host cells. Additionally, it is explored for applications in agriculture as a natural alternative to traditional pesticides, helping to protect crops from bacterial pathogens. In the medical field, it is investigated for wound healing, infection control, and treatments where conventional antibiotics are ineffective. Furthermore, research examines its potential in the food industry to prevent bacterial contamination in perishable products like meat and dairy, enhancing food safety without chemical preservatives.