Glycochenodeoxycholic acid is primarily used in medical research and diagnostics. It plays a significant role in studying bile acid metabolism and liver function. Researchers utilize it to understand conditions related to cholestasis, where bile flow from the liver is impaired. It is also employed in the development of therapeutic agents targeting bile acid-related disorders, such as primary biliary cholangitis and non-alcoholic fatty liver disease. Additionally, it serves as a reference standard in analytical techniques like HPLC and mass spectrometry for quantifying bile acids in biological samples. Its applications extend to investigating the gut-liver axis and its impact on metabolic health.