Glycolithocholic acid sodium salt is primarily used in biochemical research as a bile acid derivative. It plays a significant role in studying bile acid metabolism and its effects on the digestive system. Researchers utilize it to investigate the interactions between bile acids and cellular receptors, particularly in the liver and intestines. It is also employed in experiments exploring the regulation of cholesterol levels and the solubilization of lipids. Additionally, this compound is used in the development of diagnostic assays to assess liver function and bile acid-related disorders. Its applications extend to studying the gut microbiome and its relationship with bile acids in health and disease.