Concanamycin B is primarily utilized in biochemical research due to its potent inhibitory effect on V-ATPase, an enzyme crucial for acidifying intracellular compartments. This property makes it a valuable tool in studying cellular processes such as autophagy, lysosomal function, and intracellular trafficking. Researchers often employ it to dissect the role of pH regulation in various biological pathways, including cancer cell survival and immune response modulation. Additionally, it has been used to investigate the mechanisms of antibiotic resistance and the function of vacuolar systems in fungi and plants. Its ability to selectively disrupt V-ATPase activity provides insights into the development of targeted therapies for diseases where this enzyme plays a pivotal role.