Oligomycin C is primarily used in biochemical research to study mitochondrial function and ATP synthesis. It acts as a potent inhibitor of the mitochondrial F1F0-ATP synthase, effectively blocking ATP production. This property makes it a valuable tool for investigating cellular energy metabolism, oxidative phosphorylation, and the role of mitochondria in various physiological and pathological processes. Researchers often use Oligomycin C to induce mitochondrial dysfunction in experimental models, helping to understand diseases linked to energy metabolism, such as neurodegenerative disorders and cancer. Additionally, it is employed in studies exploring the mechanisms of apoptosis and cellular stress responses, providing insights into how cells manage energy crises.