Used primarily as a key intermediate in the synthesis of antifolate drugs, particularly in the production of certain chemotherapy agents. Its structure allows for targeted inhibition of enzymes involved in folate metabolism, making it valuable in cancer treatment research. It is also employed in the development of receptor-binding agents for diagnostic imaging and targeted therapies. Due to its functional groups, it can be conjugated to carriers for improved drug delivery. Commonly utilized in laboratory settings for creating analogs of biologically active molecules.