Hydrocupreine is primarily used in the field of medicine as an antimalarial agent. It has been studied for its effectiveness in treating malaria, particularly in cases where other treatments may be less effective. Additionally, hydrocupreine has shown potential in combating certain bacterial infections due to its antimicrobial properties. Research has also explored its use in treating protozoal infections, making it a versatile compound in the development of therapeutic drugs. Its application extends to laboratory studies where it serves as a reagent for investigating biochemical pathways and mechanisms of action in pathogens.