Used in biochemical research for studying glucosinolate metabolism in plants, particularly in Brassica species. Acts as a precursor in the synthesis of bioactive indole compounds that play roles in plant defense mechanisms. Employed in assays to investigate myrosinase enzyme activity, where it is hydrolyzed to produce signaling molecules involved in plant-pathogen interactions. Also utilized in studies exploring the health-promoting properties of dietary glucosinolates, including their potential anticancer and antioxidant effects in cellular and animal models.