Cytochalasin B is widely used in biological research to study cell motility and cytoskeletal dynamics. It inhibits actin polymerization, which helps researchers understand the role of actin filaments in processes like cell division, shape maintenance, and movement. In cell culture studies, it is employed to block cytokinesis, allowing the creation of multinucleated cells for experimental purposes. Additionally, it is used in cancer research to investigate the effects of cytoskeletal disruption on tumor cell behavior. Its ability to interfere with actin also makes it a tool in studying endocytosis and intracellular transport mechanisms.