Nebramine is primarily used in the treatment of bacterial infections, particularly those caused by gram-negative organisms. It exhibits strong antimicrobial activity and is often employed in topical formulations for skin and eye infections. Due to its efficacy in preventing bacterial growth, it is also used in ointments and solutions for burn wounds and surgical sites. In veterinary medicine, Nebramine is applied to treat ocular and dermal infections in animals. Its low systemic absorption makes it suitable for localized treatments where minimal toxicity is required.