Used primarily in veterinary medicine as an antibiotic to treat bacterial infections caused by Gram-positive bacteria, particularly in swine and poultry. It is effective against pathogens such as Staphylococcus, Streptococcus, and Clostridium species. Lincomycin B Hydrochloride is administered in feed, water, or by injection to manage respiratory, gastrointestinal, and systemic infections. Its use in animals helps improve growth performance and prevent disease outbreaks in intensive farming systems. Due to potential cross-resistance with other lincosamides, its application is often monitored to preserve efficacy and reduce the risk of antimicrobial resistance.