Anisomycin is widely used in molecular biology and biomedical research as a potent inhibitor of protein synthesis. It acts by interfering with the peptidyl transferase activity of the 60S ribosomal subunit, making it effective in studies involving gene expression and translational control. It is commonly applied in cell culture experiments to induce stress responses, including activation of MAP kinase pathways and apoptosis, allowing researchers to study cellular signaling under stress conditions. Due to its ability to cross the blood-brain barrier, anisomycin is also used in neuroscience research to investigate the role of protein synthesis in memory formation and consolidation, particularly in models of long-term potentiation and fear conditioning. In microbiology, it serves as an antimicrobial agent against certain bacteria and fungi, and is sometimes used in selective media. Its ready-made solution format ensures consistent dosing and ease of use in assays requiring rapid and reliable inhibition of protein synthesis.